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KMID : 0380620070390030299
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Korean Journal of Food Science and Technology
2007 Volume.39 No. 3 p.299 ~ p.303
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Microbiology/Fermentation/Biotechnology : Development of Immunochromatography for the Rapid Detection of Listeria monocytogenes
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Choi JIn-Gil
Kim Kwang-Yup
Ha Sang-Do
Kim Keun-Sung
Lee Kyu-Ho
Shim Won-Bo
Chung Duck-Hwa
Je Jung-Hyun
Kim Ji-Young
Kim Min-Gon
Kim Cheol-Ho
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Abstract
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The objective of this study was the development of immunochromatography (ICG) for the rapid and accurate detection of Listeria monocytogenes. Here, monoclonal antibodies (MAb) were conjugated with 40 nm colloidal gold particles, where the conjugate was used as the detection reagent in the ICG. The ICG was composed of three pads (sample, conjugate, and absorbance pads) and one nitrocellulose membrane. The colloidal gold-MAb conjugate was applied to the conjugate pad, and the test line and control line on the membrane were treated with MAb (FKLM-3BI2-37) and anti-mouse IgG, respectively. The detection limit of the ICG was 10? cell/mL and it showed no cross-reaction to food borne pathogens. We inoculated meat and lettuce samples with various counts of L. monocytogenes, and analyzed them by ICG. All the inoculated meat samples gave positive results after enrichment for 24 h in LEB. These results indicate that ICG was able to serve as a primary screening tool for L. monocytogenes in various foods and agricultural products within 20 min after enrichment.
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KEYWORD
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Listeria monocytogenes, immunochromatography, ICG, colloidal gold, monoclonal antibody
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